polydimethylsiloxane pdms substrate (Dow Corning)
86
Structured Review
Dow Corning
polydimethylsiloxane pdms substrate
Polydimethylsiloxane Pdms Substrate, supplied by Dow Corning, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydimethylsiloxane+pdms+substrate/pdms+substrates/pmc12907012-320-7-21
Average 86 stars, based on 1 article reviews
Polydimethylsiloxane Pdms Substrate, supplied by Dow Corning, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polydimethylsiloxane+pdms+substrate/pdms+substrates/pmc12907012-320-7-21
Average 86 stars, based on 1 article reviews
polydimethylsiloxane pdms substrate - by Bioz Stars,
2026-10
86/100 stars
Images
1) Product Images from "Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction"
Article Title: Pre-priming cell sheet therapy enabled by dynamic wrinkled electroactive substrate for muscle reconstruction
Journal: Bioactive Materials
doi: 10.1016/j.bioactmat.2026.01.046
Figure Legend Snippet: The fabrication and photothermal responsive behavior of the NIR-responsive dynamic wrinkled PDMS/CNTs-PPy composites. (A) The process and principle of in-situ generation of Ppy wrinkle-patterned surface. (B) Conductive atomic force microscopy (C-AFM) current mapping of PDMS/CNTs (left) and PDMS/CNTs-Ppy (right) nanocomposites. (C) It presents AFM characterization of the PDMS/CNTs-PPy composites at different time points, showing the evolution of the surface morphology from smooth to a disordered labyrinthine wrinkle pattern through 2D topography, phase, and 3D morphology images. (D–F) The evolution of width, height, and surface roughness of Ppy film with polymerization time (t), respectively. (G) Hydrophobicity change before (left) and after (right) Ppy coating. (H) Schematic of surface morphology change under NIR ON/OFF cycling. (I) Temperature profiles under NIR irradiation in different media. (J) Temperature profiles in phosphate-buffered saline (PBS) under NIR irradiation, comparing PDMS/CNTs-Ppy, PDMS/CNTs, and pristine PDMS. (K) Temperature profiles for composites with different CNT content (0, 0.025, 0.05, 0.075, and 0.1%). (L) Temperature profiles at varying NIR power densities for the 0.05 wt% CNT composite. (M) Equilibrium temperatures at different power densities. (N) Temperature cycling stability test (10 min ON/OFF intervals) under ∼1.33 W cm −2 NIR irradiation. Note: Unless specified (e.g., J-L), experiments used the 0.05 % CNT composite and ∼1.33 W cm −2 NIR power density. Data are mean ± S.D. (n = 5).
Techniques Used: In Situ, Microscopy, Irradiation, Saline
Figure Legend Snippet: The biocompatibility and functional characteristics of different surfaces. Live/dead staining (A) and CCK8 OD value (B) of C2C12 cells cultured on TCP, PDMS/CNTs, and PDMS/CNTs-Ppy. (C) Calcium transient of CMs on different surfaces at day 5 of culture. (D)Expression of cardiac-specific proteins of α-actinin (green) and CX-43 (red) in the CMs on non-electroactive PDMS/CNTs-PLA and electroactive PDMS/CNTs-Ppy. (E) and (F) Immunofluorescence intensity statistics were calculated based on CX-43 and α-actinin immunostaining images. (G)Heatmap of DEGs between PDMS/CNTs-Ppy and PDMS/CNTs-PLA after hierarchical cluster analysis (n = 3 rats per group). (H) PCA plot of proteomics data in these two groups. (I) GO terms enriched from up-regulation DEGs associated with C2C12 differentiation (PDMS/CNTs-Ppy versus PDMS/CNTs-PLA. (J) Volcano plot of protein expression in PDMS/CNTs-Ppy and PDMS/CNTs-PLA. (K) GO terms enriched from up-regulation DEGs associated with C2C12 differentiation (PDMS/CNTs-Ppy versus PDMS/CNTs-PLA). (L) Network of the muscle differentiation-related GO enriched from all DEGs with a fold change >2.0. The data were expressed as mean ± S.D. (n = 3).
Techniques Used: Functional Assay, Staining, Cell Culture, Expressing, Immunofluorescence, Immunostaining
Figure Legend Snippet: Biological activity and adhesion status of cells on PDMS/CNTs-Ppy before and after NIR irradiation. (A) Live/dead assay of C2C12 cells before (up) and after (down) cell detachment (green, live cells; red, dead cells). (B) Changes in cell morphology before (left) and after (right) NIR irradiation by SEM. (C) Immunofluorescence staining of F-actin (red) and vinculin (green). (D) Immunofluorescence staining of γ-H2AX (green). (E) Fluorescence intensity statistics were calculated based on Vinculin immunofluorescence images. (F) Fluorescence intensity statistics were calculated based on γ-H2AX immunofluorescence images. (G) Heatmap of DEGs between PDMS/CNTs-Ppy and TCP-Ppy after hierarchical cluster analysis. (H) PCA plot of proteomics data in these two groups. (I) Volcano plot of protein expression in PDMS/CNTs-Ppy and TCP-Ppy. (J) GO terms enriched from up-regulation DEGs associated with C2C12 differentiation (PDMS/CNTs-Ppy versus TCP-Ppy). (K) GO terms enriched from up-regulation DEGs associated with C2C12 adhesion and differentiation (PDMS/CNTs-Ppy versus TCP-Ppy). (L) GO terms enriched from down-regulation DEGs associated with C2C12 differentiation (PDMS/CNTs-Ppy versus TCP-Ppy). (M) Network of the cell adhesion-related GO enriched from all DEGs with a fold change>2.0. The data were expressed as mean ± S.D. (n = 3); ns means no significance.
Techniques Used: Activity Assay, Irradiation, Live Dead Assay, Immunofluorescence, Staining, Fluorescence, Expressing
Figure Legend Snippet: High integrity of cell sheets acquired by the NIR response dynamic wrinkle system. (A) Schematic illustrating the process of obtaining cell sheets, controlled remotely with on-off NIR light. (B) Immunofluorescence images of F-actin (red) and vinculin (green) in C2C12 and L6 cells. (C) Immunofluorescence images of C2C12 and L6 cell suspensions. (D) Bulk cell sheets obtained from the PDMS/CNTs-Ppy surface. (E) Viability test of cells transferred onto PDMS/CNTs-Ppy after transfer from the PDMS/CNTs-Ppy surface: Calcein-AM (green, live), EthD-1 (red, dead). (F) Immunofluorescence images of F-actin (red) and vinculin (green) in C2C12 and L6 cells before cell sheet detachment. (G) Corresponding images after detachment. (H) Immunofluorescence images of F-actin (red) and fibronectin (green) in C2C12 cell sheets. (I) Immunofluorescence images of ZO-1 (green) in C2C12 cell sheets.
Techniques Used: Immunofluorescence
Figure Legend Snippet: Schematic diagram showing the mechanism of VML tissue repair. L6 cell sheets obtained from the PDMS/CNTs-Ppy conductive wrinkled cell culture platform are transplanted into the VML site for skeletal muscle repair. The microstructured, electroactive substrate pre-primes the L6 cells, while preserving cell-cell connections, cell-ECM interactions, and a high-density, high-activity cell sheet. Consequently, the L6 cell sheets promote myogenic differentiation, maintain electromechanical coupling, reduce inflammation, and enhance neovascularisation, enabling rapid functional repair at the injury site.
Techniques Used: Cell Culture, Preserving, Activity Assay, Cell Characterization, Functional Assay
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